PicoTwist

Twisting and Stretching
Single Molecules

Adding a twist to DNA

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Paper description

5419

The elasticity of a single supercoiled DNA molecule
TR. Strick, (strick@ijm.jussieu.fr )JF. Allemand, (allemand@lps.ens.fr )D. Bensimon, (david@lps.ens.fr )A. Bensimon, V. Croquette, (vincent@physique.ens.fr )
Science (1996-03-29) 271-5257 p.1835 Science publications
Publisher : AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA. ISSN : 0036-8075
Abstract : Single linear DNA molecules were bound at multiple sites at one extremity to a treated glass cover slip and at the other to a magnetic bead. The DNA was therefore torsionally constrained, A magnetic field was used to rotate the beads and thus to coil and pull the DNA. The stretching force was determined by analysis of the Brownian fluctuations of the bead. Here, the elastic behavior of individual lambda DNA molecules over- and underwound by up to 500 turns was studied, A sharp transition was discovered from a low to a high extension state at a force of similar to 0.45 piconewtons for underwound molecules and at a force of similar to 3 piconewtons for overwound ones. These transitions, probably reflecting the formation of alternative structures in stretched coiled DNA molecules, might be relevant for DNA transcription and replication.
Corresponding Author : Strick, TR, UNIV PARIS 06,ENS,PHYS STAT LAB,24 RUE LHOMOND,F-75231 PARIS 05,FRANCE.
Affiliation(s) : (0) Laboratoire de Physique Statistique Ecole Normale SupÃ©rieure 24 rue Lhomond 75231 Paris Cedex 05;
(1) INST PASTEUR,ADN,BIOPHYS LAB,F-75015 PARIS,FRANCE.;
Key words : TORSIONAL RIGIDITY; DEPOLARIZATION; MACROMOLECULES; FLUORESCENCE; ENERGETICS; DYNAMICS
Type : Article, English. 1996-03-29
Time cited 231; Journal impact factor for year 1996 equals 23.605
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Scientific Domain: "SM Biophysics" "SM Conductivity" "Theory" "Other"
Activity: "Polymer elasticity" "Enzyme activity" "Molecular motor" "DNA motor"
Micromanipulation Technique:
"OT" "MT" "AFM" "Micro-pipette" "BFP" "Tethered part."
Visualization Technique:
"FCS" "2 photons" "TIR" "Qdots" "Epifluorecence" "FRET"
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