Single Molecules
Single Molecules |
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| 1 | Overstretching B-DNA: The elastic response of individual double-stranded and single-stranded DNA molecules SB. Smith, YJ. Cui, C. Bustamante, (carlos@alice.berkeley.edu ) Science (1996-02-09) 271-5250 p.795 Science publications Publisher : AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA. ISSN : 0036-8075 Abstract : Single molecules of double-stranded DNA (dsDNA) were stretched with force-measuring laser tweezers. Under a longitudinal stress of similar to 65 piconewtons (pN), dsDNA molecules in aqueous buffer undergo a highly cooperative transition into a stable form with 5.8 angstroms rise per base pair, that is, 70% longer than B-form dsDNA. When the stress was relaxed below 65 pN, the molecules rapidly and reversibly contracted to their normal contour lengths. This transition was affected by changes in the ionic strength of the medium and the water activity or by cross-linking of the two strands of dsDNA. Individual molecules of single-stranded DNA were also stretched giving a persistence length of 7.5 angstroms and a stretch modulus of 800 pN. The overstretched form may play a significant role in the energetics of DNA recombination. Corresponding Author : Affiliation(s) : (0) UNIV OREGON,INST MOLEC BIOL,DEPT CHEM,EUGENE,OR 97403.; (1) UNIV OREGON,HOWARD HUGHES MED INST,EUGENE,OR 97403.; (2) UNIV OREGON,DEPT PHYS,EUGENE,OR 97403.; Type : Article, English. 1996-02-09 Time cited 500; Journal impact factor for year 1996 equals 23.605 [0] ACHTER EK, 1971, BIOPOLYMERS, V10, P1625 [1] ASHKIN A, 1986, OPT LETT, V11, P288 [2] BENSIMON D, 1995, PHYS REV LETT, V74, P4754 [3] BERMAN HM, 1994, CURR OPIN STRUC BIOL, V4, P345 [4] BRESLAUER KJ, 1986, P NATL ACAD SCI USA, V83, P3746 [5] BUSTAMANTE C, 1994, SCIENCE, V265, P1599 [6] CALLADINE CR, 1992, UNDERSTANDING DNA MO [7] CALLEN HB, 1985, THERMODYNAMICS INTRO [8] DAVIDSON N, 1971, BACTERIOPHAGE LAMBDA, P56 [9] GHISLAIN LP, 1994, REV SCI INSTRUM, V65, P2762 [10] HAGERMAN PJ, 1981, BIOPOLYMERS, V20, P1503 [11] HEARST JE, 1984, Q REV BIOPHYS, V17, P1 [12] KLUG A, 1975, NATURE, V255, P530 [13] LANDAU LD, 1980, STATISTICAL PHYSIC 1 [14] LANDAU LD, 1986, THEORY ELASTICITY, P67 [15] MANNING GS, 1978, Q REV BIOPHYS, V11, P179 [16] MARKO JF, 1995, MACROMOLECULES, V28, P8759 [17] PARRA I, 1993, NAT GENET, V5, P17 [18] REES WA, 1993, BIOCHEMISTRY-US, V32, P137 [19] SAENGER W, 1984, PRINCIPLES NUCLEIC A [20] SCHELLMAN JA, 1995, BIOPHYS CHEM, V55, P95 [21] SMITH SB, 1992, SCIENCE, V258, P1122 [22] SMITH SB, 1995, BIOPHYS J, V68, A250 [23] SOBEL ES, 1991, BIOPOLYMERS, V31, P1559 [24] SVOBODA K, 1994, ANNU REV BIOPH BIOM, V23, P247 [25] THUNDAT T, 1994, NUCLEIC ACIDS RES, V22, P4224 [26] TRIFONOV EN, 1987, STRUCTURE EXPRESSION, V3, P243 [27] USSERY DW, 1992, METHOD ENZYMOL, V212, P242 [28] WEST SC, 1992, ANNU REV BIOCHEM, V61, P603 |
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