Single Molecules
Single Molecules |
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| 1 | IMAGING OF SINGLE FLUORESCENT MOLECULES AND INDIVIDUAL ATP TURNOVERS BY SINGLE MYOSIN MOLECULES IN AQUEOUS-SOLUTION T. Funatsu, (funatsu@mail.ecc.u-tokyo.ac.jp )Y. Harada, (yharada@rinshoken.or.jp )M. Tokunaga, K. Saito, T. Yanagida, (yanagida@phys1.med.osaka-u.ac.jp ) Nature (1995-04-06) 374-6522 p.555 PubMed Publisher : MACMILLAN PUBLISHERS LTD, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND. ISSN : 0028-0836 Abstract : VISUALIZATION of single actin filaments by fluorescence microscopy(1) led to the development of new in vitro assays for analysing actomyosin-based motility at the molecular level(2-5). The ability to manipulate actin filaments with a microneedle(6,7) or an optical trap(8) combined with position-sensitive detectors has enabled direct measurements of nanometre displacements and piconewton forces exerted by individual myosin molecules. To elucidate how myosin generates movement, it is necessary to understand how ATP hydrolysis is coupled to mechanical work at the level of the single molecule. But the most sensitive microscopic ATPase assay available still requires over 1,000 myosin(9). To enhance the sensitivity of such assays, we have refined epifluorescence and total internal reflection microscopies to visualize single fluorescent dye molecules. We report here that this approach can be used directly to image single fluorescently labelled myosin molecules and detect individual ATP turnover reactions. In contrast to pre,viously reported single fluorescent molecule imaging methods, which used specimens immobilized on an air-dried surface(10-12), method allows video-rate imaging of single molecules in aqueous solution, and hence can be applied to the study of man types of enzymes and biomolecules. Corresponding Author : FUNATSU, T, JRDC,ERATO,BIOMOTRON PROJECT,SENBA HIGASHI 2-4-14,MINO,OSAKA 562,JAPAN. Affiliation(s) : (0) OSAKA UNIV,DEPT BIOPHYS ENGN,TOYONAKA,OSAKA 560,JAPAN.; Key words : ACTIN-FILAMENTS; INVITRO; SUBFRAGMENT-1; MICROSCOPY; HYDROLYSIS; MOVEMENT Type : Article, English. 1995-04-06 Time cited 348; Journal impact factor for year 1995 equals 27.074 [0] AXELROD D, 1989, METHOD CELL BIOL, V30, P245 [1] BAGSHAW CR, 1993, MUSCLE CONTRACTION [2] BETZIG E, 1993, SCIENCE, V262, P1422 [3] FINER JT, 1994, NATURE, V368, P113 [4] HARADA Y, 1987, NATURE, V326, P805 [5] HARADA Y, 1988, CELL MOTIL CYTOSKEL, V10, P71 [6] HARADA Y, 1990, J MOL BIOL, V216, P49 [7] HUXLEY HE, 1990, J BIOL CHEM, V265, P8347 [8] ISHIJIMA A, 1991, NATURE, V352, P301 [9] ISHIJIMA A, 1994, BIOCHEM BIOPH RES CO, V199, P1057 [10] ISHIKAWA M, 1994, JPN J APPL PHYS, V33, P1571 [11] KISHINO A, 1988, NATURE, V334, P74 [12] KODAMA T, 1986, J BIOCHEM-TOKYO, V99, P1465 [13] KRON SJ, 1986, P NATL ACAD SCI USA, V83, P6272 [14] LINDBERG M, 1975, EUR J BIOCHEM, V53, P481 [15] LOWEY S, 1993, NATURE, V365, P454 [16] SOUTHWICK PL, 1990, CYTOMETRY, V11, P418 [17] SOWERBY AJ, 1993, J MOL BIOL, V234, P114 [18] SPUDICH JA, 1994, NATURE, V372, P515 [19] TOYOSHIMA YY, 1987, NATURE, V328, P536 [20] TRAUTMAN JK, 1994, NATURE, V369, P40 [21] YANAGIDA T, 1984, NATURE, V307, P58 [22] YANAGIDA T, 1985, NATURE, V316, P366 [23] YANAGIDA T, 1993, TRENDS BIOCHEM SCI, V18, P319 |
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