Twisting and Stretching
Single Molecules
Adding a twist to DNA
1 List
1SINGLE MYOSIN MOLECULE MECHANICS - PICONEWTON FORCES AND NANOMETER STEPS
JT. Finer, RM. Simmons, (robert.simmons@tiscali.co.uk )JA. Spudich, (jspudich@stanford.edu )
Nature (1994-03-10) 368-6467 p.113 PubMed
Publisher : MACMILLAN PUBLISHERS LTD, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND. ISSN : 0028-0836
Abstract : A new in vitro assay using a feedback enhanced laser trap system allows direct measurement of force and displacement that results from the interaction of a single myosin molecule with a single suspended actin filament. Discrete stepwise movements averaging ai nm were seen under conditions of low load, and single force transients averaging 3-4 pN were measured under isometric conditions. The magnitudes of the single forces and displacements are consistent with predictions of the conventional swinging-crossbridge model of muscle contraction.
Corresponding Author :
Affiliation(s) : (0) STANFORD UNIV,SCH MED,BECKMAN CTR,DEPT BIOCHEM,STANFORD,CA 94305.;
(1) STANFORD UNIV,SCH MED,BECKMAN CTR,DEPT BIOL,STANFORD,CA 94305.;
(2) KINGS COLL LONDON,RANDALL INST,MRC MUSCLE & CELL MOTIL UNIT,LONDON WC2B 5RL,ENGLAND.;
Key words : RABBIT PSOAS MUSCLE; KINESIN MOLECULES; SLIDING DISTANCE; SKELETAL-MUSCLE; ACTIN FILAMENT; MOVEMENT; INVITRO; FIBERS; CONTRACTION; ATP
Type : Article, English. 1994-03-10
Time cited 631; Journal impact factor for year 1994 equals 25.466
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