Twisting and Stretching
Single Molecules
Adding a twist to DNA
1 List
1DIRECT MECHANICAL MEASUREMENTS OF THE ELASTICITY OF SINGLE DNA-MOLECULES BY USING MAGNETIC BEADS
SB. Smith, L. Finzi, C. Bustamante, (carlos@alice.berkeley.edu )
Science (1992-11-13) 258-5085 p.1122 Science publications
Publisher : AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA. ISSN : 0036-8075
Abstract : Single DNA molecules were chemically attached by one end to a glass surface and by their other end to a magnetic bead. Equilibrium positions of the beads were observed in an optical microscope while the beads were acted on by known magnetic and hydrodynamic forces. Extension versus force curves were obtained for individual DNA molecules at three different salt concentrations with forces between 10(-14) and 10(-11) newtons. Deviations from the force curves predicted by the freely jointed chain model suggest that DNA has significant local curvature in solution. Ethidium bromide and 4',6-diamidino-2-phenylindole had little effect on the elastic response of the molecules, but their extent of intercalation was directly measured. Conversely, the effect of bend-inducing cis-diamminedichloroplatinum (II) was large and supports the hypothesis of natural curvature in DNA.
Corresponding Author :
Affiliation(s) : (0) UNIV OREGON,INST MICROBIOL,EUGENE,OR 97403.;
(1) UNIV OREGON,DEPT CHEM,EUGENE,OR 97403.;
Key words : GEL-ELECTROPHORESIS; FLUORESCENCE MICROSCOPY; MANIPULATION; FLEXIBILITY; DYNAMICS; BINDING; DAPI
Type : Article, English. 1992-11-13
Time cited 445; Journal impact factor for year 1994 equals 22.067
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