Single Molecules
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| 1 | INTERMOLECULAR FORCES AND ENERGIES BETWEEN LIGANDS AND RECEPTORS VT. Moy, (vmoy@newssun.med.miami.edu )EL. Florin, (florin@chaos.utexas.edu )HE. Gaub, (gaub@lmu.de ) Science (1994-10-14) 266-5183 p.257 Science publications Publisher : AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA. ISSN : 0036-8075 Abstract : The recognition mechanisms and dissociation pathways of the avidin-biotin complex and of actin monomers in actin filaments were investigated. The unbinding forces of discrete complexes of avidin or streptavidin with biotin analogs are proportional to the enthalpy change of the complex formation but independent of changes in the free energy. This result indicates that the unbinding process is adiabatic and that entropic changes occur after unbinding. On the basis of the measured forces and binding energies, an effective rupture length of 9.5 +/- 1 angstroms was calculated for all biotin-avidin pairs and approximately 1 to 3 angstroms for the actin monomer-monomer interaction. A model for the correlation among binding forces, intermolecular potential, and molecular function is proposed. Corresponding Author : Affiliation(s) : (0) TECH UNIV MUNICH,DEPT PHYS,D-85748 GARCHING,GERMANY.; Key words : SCANNING TUNNELING MICROSCOPY; ACTIN FILAMENT; STREPTAVIDIN; CELLS Type : Article, English. 1994-10-14 Time cited 330; Journal impact factor for year 1994 equals 22.067 [0] BINNIG G, 1986, PHYS REV LETT, V56, P930 [1] BINNIG G, 1987, REV MOD PHYS, V59, P615 [2] EVANS E, 1991, BIOPHYS J, V59, P838 [3] FLORIN EL, 1994, SCIENCE, V264, P415 [4] FRAUENFELDER H, 1991, SCIENCE, V254, P1598 [5] GREEN NM, 1966, BIOCHEM J, V101, P774 [6] GREEN NM, 1975, ADV PROTEIN CHEM, V29, P85 [7] HANSMA PK, 1988, SCIENCE, V242, P209 [8] HOLMES KC, 1990, NATURE, V347, P44 [9] HUTTER JL, 1993, REV SCI INSTRUM, V64, P1868 [10] ISRAELACHVILI JN, 1992, INTERMOLECULAR SURFA [11] KABSCH W, 1990, NATURE, V347, P37 [12] KISHINO A, 1988, NATURE, V334, P74 [13] LECKBAND DE, 1994, BIOCHEMISTRY-US, V33, P4611 [14] LEE GU, 1994, LANGMUIR, V10, P354 [15] MIYAMOTO S, 1993, PROTEINS, V16, P226 [16] MOY VT, 1993, REV SCI INSTRUM, V64, P403 [17] OOSAWA F, 1977, BIORHEOLOGY, V14, P11 [18] RADMACHER M, 1992, SCIENCE, V257, P1900 [19] SENDEN TJ, 1994, LANGMUIR, V10, P1003 [20] SHETERLINE P, 1994, ACTIN, V1 [21] SVOBODA K, 1993, NATURE, V365, P721 [22] WANG N, 1993, SCIENCE, V260, P1124 [23] WEBER PC, 1992, J AM CHEM SOC, V114, P3197 |
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