Single Molecules
Single Molecules |
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| 1 | DIRECT OBSERVATION OF KINESIN STEPPING BY OPTICAL TRAPPING INTERFEROMETRY K. Svoboda, CF. Schmidt, BJ. Schnapp, SM. Block, (sblock@stanford.edu ) Nature (1993-10-21) 365-6448 p.721 PubMed Publisher : MACMILLAN PUBLISHERS LTD, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND. ISSN : 0028-0836 Abstract : Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps. Corresponding Author : Affiliation(s) : (0) ROWLAND INST SCI INC,100 EDWIN LAND BLVD,CAMBRIDGE,MA 02142.; (1) HARVARD UNIV,COMM BIOPHYS,CAMBRIDGE,MA 02138.; (2) HARVARD UNIV,SCH MED,DEPT CELL BIOL,BOSTON,MA 02115.; Key words : FORCE GENERATION; INVITRO; MICROTUBULES; MOVEMENT; MOLECULES; ASSAY; SIZE; DISPLACEMENT; ORGANELLE; DENSITIES Type : Article, English. 1993-10-21 Time cited 561; Journal impact factor for year 1994 equals 25.466 [0] AMOS LA, 1974, J CELL SCI, V14, P523 [1] ASHKIN A, 1986, OPT LETT, V11, P288 [2] ASHKIN A, 1990, NATURE, V348, P346 [3] BAGSHAW CR, 1982, MUSCLE CONTRACTION [4] BLOCK SM, 1989, NATURE, V338, P514 [5] BLOCK SM, 1990, NATURE, V348, P348 [6] BLOCK SM, 1990, NONINVASIVE TECHNIQU, P375 [7] BURTON K, 1992, J MUSCLE RES CELL M, V13, P590 [8] COOKE R, 1986, CRC CRIT R BIOCHEM, V21, P53 [9] DENK W, 1990, APPL OPTICS, V29, P2382 [10] GALLAGHER NC, 1981, IEEE T ACOUST SPEECH, V29, P1136 [11] GELLES J, 1988, NATURE, V331, P450 [12] GILBERT SP, 1993, BIOCHEMISTRY-US, V32, P4677 [13] GITTES F, 1993, J CELL BIOL, V120, P923 [14] GLATTER O, 1982, SMALL ANGLE XRAY SCA [15] HACKNEY DD, 1988, P NATL ACAD SCI USA, V85, P6314 [16] HAPPEL J, 1991, LOW REYNOLDS NUMBER, P322 [17] HARADA Y, 1990, J MOL BIOL, V216, P49 [18] HARRISON BC, 1993, NATURE, V362, P73 [19] HIGUCHI H, 1991, NATURE, V352, P352 [20] HOWARD J, 1989, NATURE, V342, P154 [21] HUXLEY HE, 1969, SCIENCE, V164, P1356 [22] ISHIJIMA A, 1991, NATURE, V352, P301 [23] KAMIMURA S, 1987, APPL OPTICS, V26, P3425 [24] KAMIMURA S, 1992, J CELL BIOL, V116, P1443 [25] KAMIMURA S, 1992, J CELL BIOL, V118, P865 [26] KRON SJ, 1986, P NATL ACAD SCI USA, V83, P6262 [27] KUO SC, 1991, J CELL SCI S, V14, P135 [28] KUO SC, 1993, SCIENCE, V260, P232 [29] KUSNETSOV SA, 1986, P NATL ACAD SCI USA, V83, P8530 [30] OOSAWA F, 1986, ADV BIOPHYS, V22, P151 [31] RAY S, 1993, J CELL BIOL, V121, P1083 [32] SAKMANN B, 1983, SINGLE CHANNEL RECOR [33] SCHNAPP BJ, 1986, METHOD ENZYMOL, V134, P561 [34] SCHNAPP BJ, 1989, P NATL ACAD SCI USA, V86, P1548 [35] SCHNAPP BJ, 1990, P NATL ACAD SCI USA, V87, P10053 [36] SIMMONS R, 1992, CURR BIOL, V2, P373 [37] SIMMONS RM, 1993, MECH MYOFILAMENT SLI, P331 [38] SONG YH, 1993, P NATL ACAD SCI USA, V90, P1671 [39] SPUDICH JA, 1990, NATURE, V348, P284 [40] SQUIRE JM, 1981, STRUCTURAL BASIS MUS [41] TOYOSHIMA YY, 1990, P NATL ACAD SCI USA, V87, P7130 [42] UYEDA TQP, 1990, J MOL BIOL, V214, P699 [43] UYEDA TQP, 1991, NATURE, V352, P307 [44] VALE RD, 1985, CELL, V40, P559 [45] WANG CW, 1954, SELECTED PAPERS NOIS, P113 [46] WEINGARTEN MD, 1974, BIOCHEMISTRY-US, V13, P5529 [47] YANAGIDA T, 1985, NATURE, V316, P366 [48] YANG JT, 1990, SCIENCE, V249, P42 |
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